Small beating areas of cardiomyocytes can be seen as early as day 8, progressing to a full lawn of beating cardiomyocytes that can be harvested as early as day 15. On day 8, medium is switched to STEMdiff™ Cardiomyocyte Maintenance Medium with full medium changes on days 10, 12 and 14, to promote further differentiation into cardiomyocyte cells. On days 4 and 6, full medium changes are performed with fresh Medium C (STEMdiff™ Cardiomyocyte Differentiation Basal Medium containing Supplement C). On day 2, a full medium change is performed with fresh Medium B (STEMdiff™ Cardiomyocyte Differentiation Basal Medium containing Supplement B). The following day (Day 0), the TeSR™ medium is replaced with Medium A (STEMdiff™ Cardiomyocyte Differentiation Basal Medium containing Supplement A) to begin inducing the cells toward a cardiomyocyte fate. After one day (Day -1), the medium is replaced with fresh TeSR™ medium. Two days before the differentiation protocol, hPSC colonies are harvested and seeded as single cells at 350,000 cells/well in a 12-well format in TeSR™ medium. NOTE: This product was formerly named ‘STEMdiff™ Cardiomyocyte Differentiation Kit’ the product itself and manufacturing procedures have not changed, but the name has been updated to more accurately reflect the cell type generated.įigure 1. These cardiomyocytes can be used in various downstream applications and analyses. STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement it can be used for long-term maintenance of hPSC-derived cardiomyocytes for one month or longer. An average of 1 x 10^6 cells can be harvested from a single well of a 12-well plate. Greater than 80% of these cells will be cTnT+. This serum-free kit can be used to generate ventricular cardiomyocytes derived from a clump culture of hPSCs maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), TeSR™-AOF (Catalog #100-0401), or TeSR™-E8™ (Catalog #05990). STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (Catalog #05010) includes a medium for differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells (human pluripotent stem cells ) into ventricular cardiomyocytes (cardiac troponin T-positive ), as well as a medium for maintenance of hPSC-derived cardiomyocytes. Tissue and Cell Culture Dissociation Reagents.Work at STEMCELL View Current Opportunities >
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